Journal: Neural Regeneration Research

Article Title: Srgap2 suppression ameliorates retinal ganglion cell degeneration in mice

doi: 10.4103/1673-5374.369122

Figure Lengend Snippet: Downstream signaling pathways after inhibition of Srgap2 . (A) Detection of SRGAP2 (green, Alexa Fluor 488) expression in PC12 cells by immunofluorescence staining. n = 3 independent cell culture preparations. Scale bars: 50 μm. (B) SRGAP2 protein level was decreased after siRNA transfection and Rac1 and Cdc42 were increased after inhibiting Srgap2. (C) Quantification of B. n = 3 independent cell culture preparations. (D) mTOR signaling pathway was activated after the downregulation of Srgap2. (E) Quantification of D. n = 3 independent cell culture preparations. (F, G) ONC induced activation of the mTOR signaling pathway 7 days after ONC. Srgap2 +/– mice showed increased mTOR activation compared with WT. n = 3 independent animals in each group. Data (normalized by WT-ctrl group) are presented as mean ± SEM. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (two-tailed Student’s t -test). Cdc42: Cell division cycle 42; Ctrl: control; DAPI: 4′,6-diamidino-2-phenylindole; mTOR: mammalian target of rapamycin; ONC: optic nerve crush; Rac1: Ras-related C3 botulinum toxin substrate 1; siRNA: small interfering RNA; Srgap2: Slit-Robo GTPase activating protein 2; WT: wild type.

Article Snippet: PC12, one of the most commonly used cell lines in neuroscience research (You et al., 2022), was purchased from CHI Scientific (Jiangyin, China; RRID: CVCL_0481).

Techniques: Protein-Protein interactions, Inhibition, Expressing, Immunofluorescence, Staining, Cell Culture, Transfection, Activation Assay, Two Tailed Test, Control, Small Interfering RNA